Do there is a protocol or prompt antibodies to accomplish ICC staining on my fully differentiated ALI cultures?
As soon as the operate is complete, retrieve the Supply Cartridge from the instrument. The cell sample should be at the bottom or side of the collection tube. Note: It is recommended to spin down the Delivery Cartridge in a mini-centrifuge to get a number of seconds for comprehensive quantity Restoration.
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Function quickly at this move to pool wells right into a 15 mL tube. Be aware: For a substantial-scale dissociation, use a multichannel pipette to pool cells right into a sterile reagent reservoir. Insert the pooled wells right into a 15 mL tube.
The STEMdiff™ system presents a standardized procedure for differentiating hPSCs into epithelial cells that may be later cultured in 2D or 3D formats according to the investigate wants.
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Evaluate the results of take a look at compounds on hematopoietic stem cells with phenotypic and purposeful assessments.
Differentiation into adipocytes, osteoblasts, or chondrocytes with appropriate MesenCult™ differentiation systems
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